Detection of NNMT knockdown.
- Valentina Pozzi (353768)
- Davide Sartini (353765)
- Stefano Morganti (448973)
- Rachela Giuliante (448974)
- Giulia Di Ruscio (448975)
- Andrea Santarelli (448976)
- Romina Rocchetti (448977)
- Corrado Rubini (10527)
- Marco Tomasetti (293754)
- Giovanni Giannatempo (448978)
- Fiorenza Orlando (448979)
- Mauro Provinciali (448980)
- Lorenzo Lo Muzio (10524)
- Monica Emanuelli (353762)
Publication date
August 2013
DOIAbstract
<p>PE/CA-PJ15 cells were treated with four shRNA plasmids against NNMT (pLKO. 1-330, 1-448, 1-164, 1-711), or with transfection reagent only (mock). (A) Real-Time PCR was used to analyze the amount of NNMT mRNA in transfected compared to mock cells. (B) Protein lysates, obtained from NNMT silenced and mock cells, were analyzed for NNMT expression by Western blot.</p
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