Overexpression of NNMT attenuated apoptosis.

<p>Apoptosis was detected by flow cytometric analysis using the Annexin V-PE/7-AAD Apoptosis Detection Kit after infected for 48 h. (A) MCF-7 cells were transfected with pcDNA3.1/NNMT; (B) SK-BR-3 cells were transfected with pcDNA3.1/NNMT. The percentage of apoptosis populations was decreased in both cell lines transfected with pcDNA3.1/NNMT compared to control cells. Cells treated with staurosporine were used as a positive control. Values are expressed as means ± SD of four independent experiments. **<i>P</i><0.01 vs. control group; *<i>P</i><0.05 vs. control group.