Introduction of amino acid substitutions into SIVsmE543-3 capsid conferred virus resistance to TRIM5 restriction.

<p>Single or combinations of amino acid substitutions “P37S”, “LPA89QQ” and “R98S” were introduced into SIVsmE543-3 capsid. Single-cycle infectivity of these mutants was measured on a panel of cell lines stably expressing a TRIM5^TFP allele (Mamu-2, dark blue bars), TRIM5^Q alleles (Mamu-4, light blue bars) and TRIM5^CypA (orange bars). Infectivity was measured as percent GFP positive cells. Black bars are negative vector controls. Infectivity on this panel are shown for SIVsmE543-3 (<b>A</b>), SIVsmE543-3 S³⁷ (<b>B</b>), SIVsmE543-3 S⁹⁸ (<b>C</b>), SIVsmE5433-3 S³⁷ S⁹⁸ (<b>D</b>), SIVsmE543-3 QQ⁸⁹ (<b>E</b>), SIVsmE543-3 S³⁷ QQ⁸⁹ (<b>F</b>), SIVsmE543-3 QQ⁸⁹ S⁹⁸ (<b>G</b>) and SIVsmE543-3 S³⁷ QQ⁸⁹ S⁹⁸ (<b>H</b>).