Identification of amino acid substitutions associated with escape from TRIM5 restriction.

<p>The capsid amino acids of SIV clones from Rh444 (TRIM5^Q/Q), Rh447 (TRIM5^TFP/TFP), Rh458 (TRIM5^TFP/CypA) and Rh063 (TRIM5^TFP/CypA) were aligned to parental SIVsmE543-3. Identical amino acids were shown as dot (.), deletions are shown as dash (-). Amino acid substitutions shared among SIV clones from different macaques were highlighted with yellow. Amino acid substitutions in the CypA binding loop are highlighted in red. The critical amino acid residues identified as responsible for escape from TRIM restriction are indicated by numbers above the sequence.