Dendritic cells produce an increased pro-inflammatory cytokine profile in response to the GAG deficient mutant.

<p>Graphs show the cytokine content of culture supernatant after 6 h of infection of BMDDCs with hyphae of the indicated strains. LPS was used as a positive control, and medium as a negative control. Cytokine concentrations in culture supernatants were determined by multiplex EIA. Results are mean ± standard error of duplicate determination of cytokine concentrations, indicated in pg/mL. * indicates a significantly increased cytokine concentration induced by the Δ<i>uge3</i> mutant, as compared with the one induced by Af293, <i>p</i><0.05 by factor ANOVA. § indicates that actual value is above 5,000 pg TNFα/mL (measures exceeded upper limit of the test).