Cytokine production.

<p>(A) The levels of cytokines (TGF-β and TNF-α) were determined by enzyme immunoassay in samples of supernatants from co-cultures of macrophages with neutrophils. The tests were performed with samples obtained after 24 hours. Measurements were performed in triplicate of three different experiments. (B) Co-cultures of macrophages infected with live neutrophils and added monoclonal antibodies anti-TGF-β (10 µg/ml) or anti-TNF-α (10 µg/ml). After 7 days the parasites release were counted in a Neubauer chamber. Measurements were performed in triplicate of three different experiments. Statistical significance was determined by ANOVA. When *p≤0.05 compared to infected macrophage and +p≤0.05 compared with isotype control.