RedRif’s effect on P-glycoprotein activity – rhodamine 123 efflux ratio.

<p>P-gp activity is proportional to the ratio between Rho123 intracellular fluorescence from inhibited (+CyA) and non-inhibited (-CyA) cells. A significant increase in P-gp activity was found in RedRif-treated cells after 24 (P-gp activation effect) and 72h (P-gp induction effect) of exposure. Rif did not significantly change P-gp activity. Results refer to mean ± SD of at least 3 independent experiments performed in triplicate. Differences between treated and untreated cells were estimated using two-way ANOVA followed by Bonferroni’s multiple comparison <i>post hoc</i> test. ***p<0.001 <i>vs</i>. control.