Licopyranocoumarin and glycyrurol decreased MPP⁺-induced intracellular ROS generation.

<p>(<b>A</b>) NGF-differentiated PC12D cells were pre-incubated for 1 h with 3 µM licopyranocoumarin (LPC) or 3 µM glycyrurol (GCR), then treated with 0.3 mM MPP⁺ for 12 h. Then, the samples were loaded with 2.5 µM CM-H₂DCFDA and the fluorescence intensities were measured by flow cytometry. (<b>B</b>) The ratio of cells exhibiting ROS production was analyzed. Values are the means of four independent experiments; bars, s.d. ^##<i>p</i><0.01 compared with control cells. ^**<i>p</i><0.01 compared with MPP⁺ group cells.