Cleavage of the F0 proteins of parental and chimeric viruses.

<p>(A) Proteolytic cleavage of the F0 proteins of parental and chimeric viruses in infected DF1 cells analyzed by Western blot. (B) The positions of the precursor protein F0, the cleavage product F1, and the HN protein are indicated. Lanes: 1. rLaSota, 2. rBan/AF, 3. rLaSota-Ban/AF F, 4. rLaSota-Ban/AF HN, and 5. rLaSota-Ban/AF F HN (B) The relative levels of the F0 and F1 proteins in the Western blot experiment in part C were measured by Bio-Rad Gel Image analysis, and the efficiency of cleavage was determined by dividing the amount of F1 by the amount of F1 plus F0. Each bar represents mean and standard error of the mean of triplicate samples.