The accumulations of H₂O₂ induced by pathogens in WT<i>, atg5-1</i>, <i>rbohD</i> and <i>atg5-1</i>×<i>rbohD</i>.

<p>A. The plants (4 weeks old) were infiltrated with <i>Pst</i> DC3000 (AvrRps4) (OD₆₀₀ = 0.2) or MgCl₂ (control). DAB staining of leaves from WT, <i>atg5-1</i>, <i>rbohD</i> and <i>atg5-1 </i>× <i>rbohD</i> were taken after 24 hpi, respectively. Experiments were performed three times with similar results. B. Show are <i>Arabidopsis</i> leaves after infiltrating <i>Pst</i> DC3000 (AvrRps4) (OD₆₀₀ = 0.2) or 10 mM MgCl₂ (control) for 6 hpi. Then the changes of 525 nm peak values in fluorescence emission spectra were scanned for 120 min. Excitation wavelength: 488 nm; Excitation slit width: 10 nm; Emission slit width: 8.5 nm; Scanning speed: 200 nm/min; Scanning wavelength range: 510-550 nm.