Fold-enrichment of the membership in Gene Ontology categories for differentially expressed Tier-1 proteins under PRL-1 overexpressing conditions in HEK293 cells.

<p>The list of 172 Tier-1 proteins that were differentially expressed with a significance of q≤0.5 was submitted to the DAVID server as described in the Methods. For each cluster in the output and for each of the 3 GO classes (Biological Process, Cellular Component, and Molecular Function), the GO term with the highest fold-enrichment and an FDR-corrected significance by Fisher's exact test of q≤0.25 was selected to represent that cluster and represented here as a vertical bar. The author-ascribed description of the cluster itself is appended in parentheses to each GO term bar label on the horizontal axis. The black bar height represents the total population of proteins in a given GO term category. The white, blue, and yellow bar heights represent the number of differentially expressed Tier-1 proteins from the experiment in a given GO term category. The number shown on the bar is the fold-enrichment for a given GO term category, and the stars following each number represent the FDR-corrected significance of that fold-enrichment (no stars for 0.25≥ q>0.1, * for q≤0.1, ** for q≤0.01, *** for q≤0.001). The fold-enrichment number was calculated as (number of Tier 1 proteins in the GO category/number of Tier 1 proteins)/(number of proteins in the GO category/number of proteins in the GO class). GO = gene ontology; FDR = false discovery rate