Acute stress induces miR-135a and miR-124 downregulation in the amygdala.

<p>(A) The differential expression of mature miRNAs in the amygdala was evaluated by microarray analysis after 2 hours of restraint. The MA plot shows relative change values, expressed as log₂ ratio (stress vs control), plotted against average log intensity ((log₂Hy5+log₂Hy3)/2). (B) Levels of mature miRNAs are quantified in the amygdala RNA pool by qRT-PCR, using U6B as internal control. The statistical test used for comparison was one-way ANOVA (<i>n</i>=9). Values are means ± SE *<i>P</i> <0.001 versus naive control mice.