Acute stress increases MR protein levels in amygdala.

<p>(A) Steady state MR protein levels were measured by western blot analysis immediately after 2 hours of restraint. Lysates were obtained from pooled amygdala nuclei (<i>n</i>=4), 5 pools were generated from naive (<i>n</i>=20) and restrained mice (<i>n</i>=20). MR expression was normalized to actin signals in the same blot. Quantitative values are shown as mean ± SE <i>*P</i> < 0.05 (pairwise Student’s <i>t</i>-test). (B) qRT-PCR analysis of Nr3c2 transcript levels in naive and stressed mice (<i>n</i>=12 for both groups). Data are presented as mean ± SE. Ex 6-7 and exs 8-9 refer to the amplicons studied, corresponding to exons 6 and 7 (exs 6-7), and to exons 8 and 9 (exs 8-9) of the Nr3c2 coding sequence.