Peptides III-5 and III-6 Inhibit DNA replication.

<p><i>S. aureus</i> strain 8325-4 was grown exponentially in LB supplemented with 50 μg/ml uridine and ³H-thymidine as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072273#s4" target="_blank">Materials and Methods</a>. At T = 0 peptide III-5 (grey triangles) or III-6 (green squares) was added to a final concentration of 50 μg/ml. Addition of buffer (red filled circles) served as control. Samples were taken at the indicated time-points and incorporation of ³H into DNA was measured by liquid scintillation counting of TCA precipitated material. The experiment were repeated 3 times with similar results. The figure shows one representative experiment.