miR-200c and miR-203 target Bmi1 expression.

<p>(A) Schematic of predicted miR-200c and miR-203 sites in the human Bmi1 3′UTR broadly conserved among vertebrates; (B) miR-200c and miR-203 expression status in selected cell lines, verse MCF-7 * p<0.01; (C) miR-200c mimics and miR-203 expression vector increased miR-200c and miR-203 expression, verse control * p<0.01; (D) Inverse relationship between miR-200c and miR-203 and Bmi1 protein levels was showed; (E) and (F) Mir-200c and miR-203 suppressed the activity of the luciferase gene linked by the 3′UTR of Bmi1 respectively and a Renilla luciferase reporter for normalization. The data was obtained from three independent experiments. The mean of the results from 293T cells transfected with pMir-control and miR-200c mimics or pSlience-miR-203 were set as 1.0 respectively, * p<0.01.