MiR-200c enhances the expression of immune suppression associated genes in MDSCs.

<p>(A) Phosphorylation of STAT3, Akt and p65 in differently transfected cells. BM cells were transfected with miR-200c mimics (miR-200cM), miR-200c inhibitor (miR-200c) or control oligoes (Ctr. oligoes) and then cultured in the presence of GM-CSF and IL-6. After further culturing for 3 days, phospho-STAT3,-Akt and-p65 were analyzed by Western blot. (B) Expression of immuno-suppression associated genes in CD11b⁺Gr-1⁺ cells isolated from spleens of mice with Lewis or B16 tumors. Ctr., CD11b⁺Gr-1⁺ cells isolated from spleens of tumor-free mice. (C and D) Expression of immune suppression associated genes in differently transfected BM cells. BM cells were transfected with miR-200c mimics (miR-200cM), miR-200c inhibitors (miR-200cI), or control oligoes (Ctr. oligoes) and then cultured with GM-CSF and IL-6. After further culturing for 3 days, immunosuppressive genes were detected by qRT-PCR (C) and Western blot (D). Each experiment was independently performed three times and for Western blotexperiments, the representative results are shown here. *, p<0.05; **, P<0.01