<i>slk19^3R</i> causes premature movement of Cdc14 from the nucleus to the cytoplasm at the end of anaphase.

<p>Live cell microscopy time course montages of representative Cdc14-GFP and mCherry-Tub1 in (A) <i>SLK19</i> and (B) <i>slk19^3R</i> cells. The appearance of Cdc14-GFP at the bud neck is indicated with arrows, and spindle disassembly is indicated with arrowheads. For the <i>slk19^3R</i> condition in (B), the “0 min” time point was set as the image frame immediately prior to the appearance of Cdc14-GFP at the bud neck. As spindle disassembly occurred during the “10 min” time frame in <i>slk19^3R</i>, a corresponding 10-min time period was depicted in the <i>SLK19</i> condition in (A).