Model of the proposed activities of Slk19 and Slk19^3R during anaphase with respect to the regulation of Cdc14 localization.

<p>In early anaphase, the FEAR network is activated to release Cdc14 from the nucleolus to the nucleus, and both Slk19 and Slk19^3R are proficient at promoting the release of Cdc14 from the nucleolus (diagram, left side). In late anaphase, wild type Slk19 retains Cdc14 within the nucleus until after spindle disassembly, while Slk19^3R cannot retain Cdc14 within the nucleus, resulting in the premature release of Cdc14 to the bud neck in the cytoplasm prior to spindle disassembly (diagram, right side). MEN proteins, which are involved in the release of Cdc14 from the nucleus to the bud neck during mitotic exit, might directly oppose Slk19 in late anaphase and might be more effective at promoting the release of Cdc14 to the bud neck in the presence of the mutant Slk19^3R protein. Alternatively, Slk19 might retain Cdc14 in the nucleus by a mechanism that not indirect opposition of the MEN, and mutant Slk19^3R protein is unable to retain Cdc14 within the nucleus until the end of anaphase.