Cell-cell junctions of HUVECs.

<p>Seventy-two hours after the siRNA treatment, immunostaining for ZO-1 and VE-cadherin, components of tight junctions and adherence junctions, respectively, in HUVECs treated with control siRNA or VASH1 siRNA was performed. Bar = 50 µm. B: Protein content of ZO-1, VE-cadherin, VASH1, and β-actin in HUVECs treated with control siRNA or VASH1 siRNA was analyzed by Western blotting. C: HUVECs were transfected with the VASH1 expression vector, and stealth siRNA. The cells were extracted 48 hours later, and Western blot analysis was performed.