Silencing striatin in HUVECs inhibites cell migration.

(A) HUVECs were transfected with scrambled siRNA or striatin targeted siRNA 1, 2 or 3 for 48h. Striatin densitometry values were adjusted to actin intensity, then normalized to expression from the control sample. Expression in CON group was normalized to 1, *p<0.01 versus CON. Bars represent SD, n = 4. (B) After transfection assays, HUVECs were scrapped to create a cell-free (wounded) area. Cells were incubated with 10nM E2 or with solvent as control for another 24h. Migration was monitored. Representative images of cell migration are shown. Gap distance in CON group was normalized to 1, *p<0.01 versus CON. Bars represent SD, n = 6. (C) After transfected with striatin siRNA1, 2, 3 or scrambled siRNA for 24h, transwell experiments were performed. Representative images of transwell experiments are shown. The number of the invaded cell in the lower surface of the membrane in CON group was normalized to 1, *p<0.01 versus CON. Bars represent SD, n = 6.