Increased fibrosis and EMT in the irradiated liver.

<p>(A) QRT-PCR analysis of liver mRNA from radiation-treated mice for tfg- β, α -sma, collagen a1, n-cadherin, s100a4, and bmp7(n ≥3 mice/group). Mean±SD results are graphed. (B) & (C). Western blot analysis of TGF-β (25 kDa: processed form) (inducer of fibrosis) and α-SMA (fibrogenic marker) expression (GAPDH was used as an internal control) (n≥3 mice/group). Data shown represent one of three experiments with similar results (B: Immunoblot/C: Band density of TGF-β and α-SMA). Data represent the mean ± SD of three independent experiments. (D) Sirius red staining in liver sections from representative control and irradiated mice (X40) (*p<0.05, vs own control group).