TLR9 agonist and recombinant cytokines promote increase in anti-apoptotic Bcl-2 protein in ASC.

<p>The intracellular content of Bcl-2 was analyzed in terms of mean fluorescence intensity (MFI) ± SD by flow cytometry in CD138-positive ASC derived from CD19-positive B cells of control- or V<i>Tn</i>-immunized mice. Histogram is representative of 3 experiments (<i>A</i>). The dashed line represents the MFI of Bcl-2 in purified CD19-positive B cells from control mice cultured in medium under basic conditions. The percentage of positive cells was analyzed in peritoneal (<i>B</i>), splenic (<i>C</i>) or medullar cells (<i>D</i>). #<i>p</i> < 0.05 compared to CD19-positive B cells from V<i>Tn</i>-immunized mice in medium under basic conditions.