B cells upregulate CD86 and CD69 independently of TLR7.

<p>Wild-type (WT) or TLR7-deficient mice were infected with FV. At 7 dpi, splenocytes were harvested and the levels of CD86 (<b>A</b>) and CD69 (<b>B</b>) expression determined for B cells (CD19+) by flow cytometry. A representative flow cytometry plot for the CD19+ gated cells for each group is shown. Each bar of the histogram represents the average of 5–6 mice. Control ‘naïve’ mice were inoculated with an equivalent dose of spleen homogenate without FV.