Analysis of the electron micrograph of fascin- and α-actinin-F-actin rafts.

<p>(A) Unprocessed electron microscope images of actin rafts created with α-actinin (top) or fascin (bottom). (B) Same images transformed with a fast Fourier transform (FFT) filter, a line scan (red line) analysis of the intensities. (C) Intensity profile from the line scan in (B). The data were fit with sum of Gaussians (red lines). The peaks represent the position of actin filaments in the rafts. (D) Histogram of the center-to-center distance between actin filaments with fascin (closed circle) and α-actinin (open circle) analyzed with single Gaussian distribution (solid line). The average distances of fascin- and α-actinin-F-actin rafts were 12.5 ± 2.3 nm (n = 78, mean ± S.D.) and 39.7 ± 16.5 (n = 97, mean ± S.D.), respectively.