Foxa1 increases the median MMTV-Ras transcriptional response and decreases cell-to-cell variability in the FISH signal intensity.

<p>(A) Gating of sub-groups of cells representing no Foxa1 expression, low and high Foxa1 expression used for in-depth analyses of MMTV transcriptional variability. (B) A gallery of representative images of cells within the three sub-groups showing different degrees of Foxa1 immunofluorescence. (C, D) Box-and-whisker plots of the RNA FISH signal intensity (mean pixel intensity units) and size (µm) distributions within each sub-group. (E) Computation of the individual cell-to-cell variability in the FISH signal intensity using the % CV. Each % CV is calculated from greater than 100 cells. The % CV shown represents the mean + SEM from 4 independent experiments. *, indicates a significant difference from the Foxa1 negative (-) sub-group as determined by the Mann-Whitney U Test (p< 0.05).