The signal of <i>EIF5A2</i>, <i>MYCN,</i> and <i>MCL1</i> genes is decreased in HU and GEM treated UACC-1598.

<p>A. Genes <i>EIF5A2</i>, <i>MYCN</i>, and <i>MCL1</i> are present on DMs in UACC-1598 cells. Metaphase spread of UACC-1598 cells detected with DNA probe for <i>EIF5A2</i>, <i>MYCN</i>, and <i>MCL1.</i> For the overlapped pictures, <i>EIF5A2</i> and <i>MCL1</i> signals are shown in red, and <i>MYCN</i> signal is shown in green. Overlapped <i>EIF5A2</i>/<i>MYCN</i> and <i>MCL1</i>/<i>MYCN</i> are shown in yellow. B. Representative pictures of <i>EIF5A2</i>/<i>MYCN</i> and <i>MCL1</i>/<i>MYCN</i> FISH signals in interphase cells of UACC-1598 and how individual cells are separated into different groups. <i>MYCN</i> signal is shown in green, <i>EIF5A2</i> and <i>MCL1</i> shown in red, and the overlap is shown in yellow. The percentage of cells in Groups 1, 2, and 3 is based on <i>EIF5A2</i>/<i>MYCN</i> and <i>MCL1</i>/<i>MYCN</i> FISH signals. The statistical analysis showed the differences of cells in Group 1 and Group 2/3 compared to the control cells. *denotes a <i>P</i> value of 0.01 to 0.05 and **denotes a <i>P</i> value of 0.001 to 0.01.