Six-OHDA leads to Nox activation.

<p>Effect of 6-OHDA on mRNAs of Nox1 (A), Nox2 (B) and Nox4 (C) isoforms in the striatum. HPRT was used as an internal control; n=6. The statistical significance is expressed as: * P < 0.05. In D and I, representative Western blots illustrating p67^phox protein content in cytosolic and membrane fractions obtained from striatum and SN respectively, after 6-OHDA-induced PD. Six-OHDA did not induce significant p67^phox translocation from the cytosol to the plasma membrane in none of the analyzed samples; n=4 tested samples. In E and J, effect of 6-OHDA on striatal and SN Rac-1 total protein levels. The graphs represent the mean ratio of Rac-1 densitometric data in relation to β-actin of 5 tested samples. Significance compared to Wt saline at **p <0.01. In F, effect of 6-OHDA on mRNAs of Nox1 (F), Nox2 (G) and Nox4 (H) isoforms in the SN. HPRT was used as an internal control; n=6. The statistical significance is expressed as: * P < 0.05.