Effect of <i>P. falciparum</i> 3D7 GFP infection on surface MHC class I expression.

<p>Primary human hepatocytes were infected with <i>P. falciparum</i> 3D7 GFP sporozoites and surface MHC class I expression was assessed in GFP+ and GFP- cell populations at days 5 and 8 post-infection using immunostaining followed by flow cytometry (for details see Materials and Methods). (A) Dot plots (gray) show side scatter characteristics of GFP- cells (uninfected), while the contour plots (black) show GFP+ (infected) populations at day 5 and day 8 post-infection. Filled histograms represent staining with an isotype control antibody; solid lines, staining with a MHC class I-specific antibody in both populations at day 5 and day 8. Data are representative of two independent experiments. (B) Modulation of MHC class I in <i>P. falciparum</i> 3D7 GFP infected primary hepatocytes on day 5 post-infection by recombinant IFNγ and TNFα supplied at day 3 post-infection. Dot plots (GFP-) represent populations of uninfected cells, while contour plots show infected (GFP+) cells. Numbers are MFI of specific fluorescence obtained following staining with an APC-conjugated isotype control or MHC class I-specific antibody. (C) Expression of MHC class I in <i>P. falciparum</i> 3D7 GFP infected primary hepatocytes on day 5 post-infection in response to AS (10% v/v) supplied on day 3. Filled histograms show staining obtained with an isotype control antibody, solid lines - staining obtained with an MHC class I specific antibody. Numbers represent MFI values of cell populations stained for MHC class I expression.