The effect of isoalantolactone on the cell cycle in UM-SCC-10A cells.

<p>(A) The DNA content in each cell cycle phase in UM-SCC-10A cells was analyzed by flow cytometry. The representative histograms are from UM-SCC-10A cells incubated with different concentration of isoalantolactone (25 and 50 µM) or caspase inhibitor (Z-VAD-FMK50 µM). (B) Isoalantolactone treatment induced a dose-dependent increase in the proportion of cells in the G1 phase and a decrease in cells in the S and G2 phases compared to the control. Z-VAD-FMK treatment did not prevent cell cycle arrest following high-dose isoalantolactone treatment. The results are represented as the mean ± SEM for three independent experiments with similar results. *<i>P</i><0.05 and **<i>P</i><0.01 compared to the control.