LGG+Bb12 colonization effect on frequencies and tissue distribution of TLR expressing MNCs.

<p>(<b>a</b>) TLR2, (<b>b</b>) TLR4 and (<b>c</b>) TLR9 expression pre-challenge and (<b>d</b>) TLR3 expression post-challenge by MNCs of Gn pigs. Frequencies of blood MNCs expressing TLRs were very low (<0.4%) and are not shown. Post-challenge TLR3 expression by MNCs from vaccinated pigs (Pro+Vac and Vac) was undetectable. Letters A and B indicate significant (p<0.05) difference between total bacterial numbers of Pro+Vac and Vac groups. Symbols † and † † indicate significant (p<0.05) difference between Pro and Control groups. Asterisk (<b>c</b>) indicates that frequencies of TLR9 expressing MNCs were significantly (p<0.05) higher for Pro+Vac compared to Vac, Pro and Control pigs.