Technical features of primary astrocytes culture and MTO fluorescence.

<p>(<b>A</b>) Triple staining of astrocytes in culture. Astrocytes were pre-stained with MTO, then fixed and stained with antibodies against GFAP and with nuclear dye DAPI. (<b>B</b>) The intensities of MTO fluorescence over time. Graph represents relative average intensities of MTO signal of live control cells and cells exposed to continuous 3 mM H₂O₂ (n = 25). Time frame of treatment with H₂O₂, MTO staining, post-wash recovery and acquisition were the same as in experiments with continuous and fluctuating exposure to H₂O₂.