MeHg increases ROS in astrocytes.

<p>(A) To assess .O₂⁻ production, astrocytes were exposed to hydroethidium and then treated with 10 µM MeHg for 20 min. Images are representative of 3 independent experiments. (B) H₂O₂ production was assessed by loading. astrocytes with 50 µM 2′,7′-dichlorodihydrofluorescein for 30 min. Astrocytes were then treated with 5, 10, and 20 µM MeHg for 1 h and DCF fluorescence was measured. Results are depicted as % DCF fluorescence in control astrocytes. Results are mean ± SEM from 4 separate astrocyte preparations. *p<0.05 vs. control.