Increased production of HGF by HMFs in 3D conditions.

<p>(A) An ELISA assay showing the concentration of HGF [pg/ml] in different conditioned media collected from three 2D conditions (bare, collagen-coated, mixed matrix-coated), and two 3D conditions (collagen I only, mixed matrix). HMF cells in all 3D conditions significantly increased the secretion of HGF. ‡ represents a p value of less than 0.05. (B) Invasion of MCF-DCIS cells with HGF neutralizing antibody (anti HGF) using transwells. The HGF neutralizing antibody (0.5 μg/ml) is added to various 2D CM, 3D CM and BK CM (the conditioned medium collected from blank mixed gels). A serum free medium is used as a negative control and a 20% serum-containing medium is used as a positive control. ‡ represents p value of less than 0.05. (C) The aspect ratio of MCF-DCIS cells cultured in a 3D condition with 2D CM and 3D CM with or without the HGF neutralizing antibody. ‡ represents a p value of less than 0.05.