Non-homologous peptides induce CD4⁺ and CD8⁺ T-cell responses.

<p>A: CD4+ T-cells were isolated from spleens of mice immunized with single peptides as indicated. T-cells were restimulated for 48h in the presence of unloaded control APC (w/o) or APC loaded with the respective NHP used for immunization. Peptides not used for immunization served as controls. IFNγ secretion was measured by ELISA of culture supernatant. Representative results from three independent experiments are shown. B: CD8⁺ T-cells were restimulated in a similar fashion. Only NHP-B immunization/restimulation resulted in IFNγ secretion. C: Splenocytes from NHP-B immunized mice were restimulated with long peptides and a predicted short H2D^b epitope (NHP-B2, 10mer, SSPQGSPEPL) derived from NHP-B. IFNγ secretion was measured by ELISA of culture supernatants (representative example of one out of three independent experiments using 3 animals per group).