YF-17DD Immunodominant HLA-A*0201 restricted epitope in the NS4b protein.

<p><b>A</b>. Sequences of the peptides used to identify the immunogenic nonamer epitope in NS4b. IC₅₀ values for IEDB-AR prediction were calculated; <b>B</b>. PBMCs from HLA-A2 vaccinees were cultured in the presence or absence of 9–10mer (NS4b_77–85, NS4b_76–84, NS4b_75–83, NS4b_75–84) peptides. ELISPOT assay; <b>C.</b> Intracellular cytokine staining were performed. All peptides analyzed could activate CD8⁺ T-cells and the expansion of NS4b_76–84-specific CD8⁺ T-cells was the highest among the four peptides tested. Plots gated on CD8⁺ T-cells for one representative donor are shown.