PHF8 inhibits ESCC cell apoptosis.

<p>ESCC cells stably expressing PHF8 or non-silencing shRNA, or control cells were stained with annexin V-PE and 7-AAD, and then analyzed by an LSRFortessa. Annexin V-PE is a sensitive probe for identification of apoptotic cells, whereas 7-AAD is a standard flow cytometric viability probe used to detect non-viable cells. Cells that stain positive for both annexin V-PE and 7-AAD are either in late apoptosis or are non-viable (Q2). Early apoptotic cells stain positive for annexin V-PE and are negative for 7-AAD (Q4). Data are representative of three independent experiments.