G_α16 regulates Wnt7a/Fzd9-mediated PPARγ activation.

<p>NSCLC cell lines, H157 (A) or H2122 (B) cells were transfected either with control siRNA or G_α16-specific siRNAs together with PPAR-RE-luciferase reporter and either without or with Wnt7a expression vector. After 48 h, the lysates were assayed for luciferase activities as described in the Methods. Data represents mean ± SEM of three separate experiments. **, <i>p</i><0.01; versus empty vector control. ^##, <i>p</i><0.01; versus Wnt7a. NSCLC cell lines, H157 (C) or H2122 (D) cells were transfected either with empty vector or constitutively active G_α16 Q212L or G_αo Q205L expression vectors together with PPAR-RE-luciferase reporter. After 48 h, the lysates were assayed for luciferase activities as described in the Methods. Data represents mean ± SEM of three separate experiments. **, <i>p</i><0.01; versus empty vector control. NSCLC cell lines, H157 (E) or H2122 (F) were transfected with either empty vector or constitutively active G_α16Q212L. After 24 h, the cells were treated either with or without PPARγ inhibitor (T0070907, 10 µM) as described in the Methods. Cell proliferation rates were later determined using an MTS assay as described in the Methods. Data represents mean ± SEM from three independent highly reproducible experiments. ^##, <i>p</i><0.01; versus empty vector control. **, <i>p</i><0.01; versus G_α16 Q212L+T007090.