Loss-of-function of Fzd5 alters the polarity of hippocampal neurons.

<p><b>A</b>. Immunodetection of the axonal marker MAP1B-P and the somato-dendritic marker MAP2 in neurons 48 h after transfection with the scramble or shFzd5 vectors. GFP was used to detect transfected neurons. Representative images, shown in the upper panels, of a control neuron expressing the axonal marker distributed through the axon (arrows) and in the lower panels, a neuron transfected with the shFzd5 expressing no axonal marker (arrows). Scale bar: 20 µm. <b>B</b>. Quantification of transfected neurons with polarized axonal marker. Polarization of axonal markers was significantly lower in neurons transfected with the shFzd5 vectors compared to that of control neurons. Bars represent mean ±SEM of three independent experiments. * p< 0.05.