Fzd5 overexpression in neurons that have already developed the axon alters the morphology of hippocampal neurons.

<p><b>A</b> Immunodetection of transfected (GFP+) hippocampal neurons with the axonal marker Tau1 (upper panels) and the somato-dendritic marker MAP2 (middle panels) in neurons transfected with the empty vector or overexpressing Fzd5 at 3 DIV. In the lower panels a merge of both markers with GFP is shown. <b>B</b>-<b>D</b>. Quantification of axonal length (D), total axonal branching (E), and total dendritic length (F). Neurons overexpressing Fzd5 with polarized axonal markers distribution showed a significant decrease in axonal length and a significant increase in axonal branching and dendritic length, compared to control neurons. <b>E</b>. Quantification of primary, secondary and tertiary dendritic branching. Neurons overexpressing Fzd5, with non-polarized axonal markers, showed a significant increase in secondary and tertiary dendritic branching compared to control neurons. Polarized neurons overexpressing Fzd5 showed significantly more tertiary branching than control neurons. <b>F</b>. Quantification of branching points of the neurites. Neurons overexpressing Fzd5 showed a significant increase in branching points compared to control neurons. Scale bar: 20 µm. Bars represent mean ±SEM of three independent experiments. * p< 0.05; ** p< 0.01; *** p< 0.001.