RNAP II CTD phosphoserine-2 levels decreased with increasing amounts of DRB.

<p>HeLa cells were mock infected or infected with WT HSV-1 KOS at an MOI of 10. DRB stocks were prepared in Dimethyl formamide. At 1, 3 and 5 h after infection, Dimethyl formamide alone (D) or increasing amounts of DRB from 0 to 100µµM were added to cell monolayers. At 8 h after infection, whole cell lysates were prepared and separated on 5-15% linear gradient SDS-polyacrylamide gels. Western blots were probed with polyclonal antibody N20, which detects all forms of RNAP II; monoclonal antibody H5, which detects phosphoserine-2, and monoclonal antibody H14, which detects phosphoserine-5. For HSV-1 proteins, antibody P1011 was used to detect IE protein ICP4, P1119 for ICP27, P1123 for gB and P1103 for gD. Lamin A/C served as a sample loading control.