SB-3CT suppressed microglia activation and astrogliosis 7 days post-trauma.

<p><b><i>A.</i></b> A representative microphotograph showing the cortical area for glial cell activation analysis. <b><i>B.</i></b> photomicrographs of cortical region in coronal sections of mouse brain immunofluorescently stained with microglial marker CD11b and astrocytic marker GFAP showing increased astroglial activity 7 days post-trauma. SB-3CT treatment attenuated activation of microglia and astrocytes in the lesioned cortex. Scale bar = 50 µm. <b><i>C.</i></b> Numbers of the CD11b positive microglia were counted from a total of approximately 1600 cells in the area showed in <b><i>A</i></b><b>.</b> The number of CD11b positive microglia was significantly lower in the lesioned cortex of SB-3CT-treated mice compared to vehicle-treated animals; n = 3 for each group; *, p<0.05 by one-tailed, unpaired Student’s <i>t</i>-test. Data are expressed as mean ± SEM.