Effects of PEG8000 on the expression of genes controlling H₂S generation and on H₂S production rate in WT seedlings.

<p>(<b>A</b>) Expression detection of H₂S generating critical enzymes coding genes in WT seedlings treated with 0.1 g ml⁻¹ PEG8000 for 0, 1, 2, 4, 8 h; (<b>B</b>) Expression detection of H₂S generating critical enzymes coding genes in WT seedlings treated 2 h with 0, 0.05, 0.1, 0.2, 0.4 g ml⁻¹ PEG8000. <i>EF1-α</i> was used as an internal control of RT-PCR; (<b>C</b>) Endogenous H₂S production rate of WT seedlings treated with 0.2 g ml⁻¹ PEG8000 for 2 h. Results are shown as mean ± SE (n = 3 independent experiments). Letter numbers indicate significant differences between treatments and substracts (<i>P<0.05</i>).