Expression pattern of the <i>BnNF-Y</i> genes in plants subjected to drought stress.

<p>The expression of <i>BnNF-YA</i> (A), <i>BnNF-YB</i> (B), and <i>BnNF-YC</i> (C) genes in the leaves and roots of plants exposed to 15% (w/v) PEG6000. Three-week-old canola seedlings were treated with 15% (w/v) PEG-6000 for 1 and 3<b> </b>h. Total RNA was extracted from leaves (L) and roots (R) for quantitative PCR (qRT-PCR) analysis. Transcript levels of each BnNF-Y were first normalized to those of the housekeeping gene 18S and then compared to each time-point control. Each data point represents the mean ±SE of three independent experiments. Significant differences between treated samples and untreated controls (same tissue only) are indicated by single (P<0.05) or double (P<0.01) asterisks, according to Dunnett’s method of one-way ANOVA in SPSS. Expression levels in untreated samples (CK, 1-h or 3-h leaf and root samples) were arbitrarily set to 1.0.