Resveratrol treatment activates AMP-activated protein kinase (AMPK) in mouse cortical collecting duct mpkCCD_c14 cells.

<p>Polarized mpkCCD_c14 cells were fed with media containing resveratrol or vehicle control for the times indicated. Cell extracts (50 µg protein) were separated on a 4–12% SDS gel. (<i>A</i>). Representative immunoblot analysis of the time-dependence of resveratrol-mediated AMPK activation, as measured by immunoblotting of phosphorylated AMPKα (p-AMPKα) in polarized mpkCCD_c14 cells. (<i>B</i>). Density of p-AMPKα bands were quantified and normalized to protein loading using β-actin as control, and means ± SEM were plotted (<i>n</i> = 3), *<i>p</i><0.01 relative to vehicle control at same time point.