Consequences of CNS-specific Sox10 deletion on the expression of marker proteins of differentiating OL.

<p>(<b>A–X</b>) Immunohistochemistry was performed on transverse spinal cord sections from the forelimb region of wildtype (wt) (<b>A–C,G–I,M–O,S–U</b>) or <i>Sox10^ΔCNS</i> (ko) (<b>D–F,J–L,P–R,V–X</b>) mice at P3 (<b>A,D,G,J,M,P,S,V</b>), P7 (<b>B,E,H,K,N,Q,T,W</b>) and P14 (<b>C,F,I,L,O,R,U,X</b>) using antibodies directed against Myrf (<b>A–F</b>), CC1 (<b>G–L</b>), CNPase (<b>M–R</b>), and Nkx2.2 (<b>S–X</b>). Ventral horn region is shown. Scale bar, 75 µm. (<b>Y,Z</b>) From these stainings, the total number of Myrf-positive (<b>Y</b>) and Nkx2.2-positive cells in the white matter (<b>Z</b>) was quantified in wildtype (black bars) and <i>Sox10^ΔCNS</i> (white bars) mice. At least 9 separate sections from the forelimb region of 3 independent specimens were counted for each age and genotype. Data are presented as mean ± SEM for biological replicates. Differences to the wildtype were statistically significant for oligodendroglial cell numbers between wildtype and mutant from P3 onwards as determined by the Student's <i>t</i> test (*, P≤0.05; ***, P≤0.001).