Quantification of CHIKV-118-GFP-infected cells by imaging.

<p>Acquired confocal images of CHIKV-118-GFP infection in HuH-7 cells analyzed using the customized plugin in the Image Mining platform. From the merged image (A), the regions of individual nuclei from the DAPI channel (B) are defined using a watershed algorithm (C). The resulting output shows each individual nucleus with distinct color (A color map is drawn in frame C). A positive signal from the GFP channel (D) is determined using a threshold value to generate the GFP foreground (E). CHIKV-infected cells are identified by the overlap between nuclei identified in C and GFP foreground in E (F) and the percentage of nuclei of infected cells is defined by the ratio between nuclei in F and C. (false-color imaging were applied on panels A, B, and C).