<p>First, variations in illumination and staining are corrected. Nuclei are identified by thresholding, then used as seeds to identify cell edges. Finally, DNA-damage foci are identified. Schematic data shown, based on image courtesy of Scott Floyd, Michael Pacold, and Michael Yaffe. Colors of nuclei, cells, and foci are arbitrary.</p
Digital image analysis has demonstrated to be a very promising tool to promote toxicity testing and ...
With the contiguous shift of biology from a qualitative toward a quantitative field of research, digi...
The advent of fluorescent proteins, together with the recent development of advanced high-resolution...
<p>Panel A. Using a Sytox green staining, all nuclei are first detected based on a local-maxima thre...
<p>(A) the original raw micrograph, (B)–(D) show the respective detection results superimposed on th...
<p>(A) the original raw micrograph, (B)–(D) show the respective detection results superimposed on th...
(A) An example image of nuclei stained with Hoescht dye. The scale bar is 500 μm. These images were ...
<p>Acquired confocal images of CHIKV-118-GFP infection in HuH-7 cells analyzed using the customized ...
There are many methods to analyze cell nuclei, but few can detect the presence of DNA damage, withou...
`These authors contributed equally. Key words: confocal microscopy, image quantification, nucleus, p...
The vast majority of microscopic data in biology of the cell nucleus is currently collected using fl...
<p>(A) Spread nuclear DNA from budding yeast meiosis stained with DAPI (DNA, left) and antibodies ag...
(Upper panel) schematic representations of the stages of image analysis. Text boxes contain brief de...
<p>γH2AX focus analysis was assessed in PBMCs immediately, 1 h and 20 h after indicated exposure con...
<p>Alterations in nuclear area A) and cytosolic area B) in cells treated with 5 ng/mL doxorubicin or...
Digital image analysis has demonstrated to be a very promising tool to promote toxicity testing and ...
With the contiguous shift of biology from a qualitative toward a quantitative field of research, digi...
The advent of fluorescent proteins, together with the recent development of advanced high-resolution...
<p>Panel A. Using a Sytox green staining, all nuclei are first detected based on a local-maxima thre...
<p>(A) the original raw micrograph, (B)–(D) show the respective detection results superimposed on th...
<p>(A) the original raw micrograph, (B)–(D) show the respective detection results superimposed on th...
(A) An example image of nuclei stained with Hoescht dye. The scale bar is 500 μm. These images were ...
<p>Acquired confocal images of CHIKV-118-GFP infection in HuH-7 cells analyzed using the customized ...
There are many methods to analyze cell nuclei, but few can detect the presence of DNA damage, withou...
`These authors contributed equally. Key words: confocal microscopy, image quantification, nucleus, p...
The vast majority of microscopic data in biology of the cell nucleus is currently collected using fl...
<p>(A) Spread nuclear DNA from budding yeast meiosis stained with DAPI (DNA, left) and antibodies ag...
(Upper panel) schematic representations of the stages of image analysis. Text boxes contain brief de...
<p>γH2AX focus analysis was assessed in PBMCs immediately, 1 h and 20 h after indicated exposure con...
<p>Alterations in nuclear area A) and cytosolic area B) in cells treated with 5 ng/mL doxorubicin or...
Digital image analysis has demonstrated to be a very promising tool to promote toxicity testing and ...
With the contiguous shift of biology from a qualitative toward a quantitative field of research, digi...
The advent of fluorescent proteins, together with the recent development of advanced high-resolution...
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