Stacked area charts of the P_194-208 synthetic peptide digested with purified ERAPs.

<p>The P_194-208 synthetic peptide (sequence indicated at the top of the figure and the 11-mer identified by MS is bolded) was digested at different times with ERAP enzymes as follows: ERAP1 at an E/S ratio of 1:800 (panel A), ERAP2 at an E/S ratio of 1:800 (panel B), both ERAP1 and 2 at an E/S ratio of 1:800 (panel C), and both ERAP1 and 2 at an E/S ratio of 1:1600 (panel D). The results depicted as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079596#pone-0079596-g002" target="_blank">Figure 2</a> are the mean values of three or four independent experiments.