Stacked area charts of M_163-177 and NS2_31-45 synthetic peptides digested with purified ERAPs.

<p>M_163-177 (panels A-D) and NS2_31-45 (panels E-H) (sequences are indicated at the top of the figure and the respective HLA ligands identified by MS are bolded) synthetic peptides were digested at different times with ERAP enzymes as follows: ERAP1 at an E/S ratio of 1:800 (panels A and E), ERAP2 at an E/S ratio of 1:800 (panels B and F), both ERAP1 and 2 at an E/S ratio of 1:800 (panels C and G), and both ERAP1 and 2 at an E/S ratio of 1:1600 (panels D and H). The intensity peaks obtained by MALDI-TOF analysis for all peptides at each time point were added and taken as 100% for each time point and depicted. The different N-end trimming products detected are named in their respective region. The results depicted are the mean values of three or four independent experiments.