Effect of SAM and SIN on the steady state kinetics of cleavage activity.

<p>Steady state kinetics experiments of RM.BpuSI cleavage activity were carried out by incubating 200 nM of purified RM.BpuSI protein with 1.25 to 25 μM of FAM-labeled sub01 at 37°C for 1 h in the presence or absence of 160 μM of SAM or SIN. The initial rate (v) for each substrate concentration was plotted against substrate concentration. K_m and V_max values were obtained by fitting the data points to the Michaelis-Menten equation. Error bars indicate the standard deviation of the fitting of the initial linear rate of each data point.